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yflL [2019-09-05 10:57:43]
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yflL [2019-09-05 10:57:43]

acylphosphatase
locus
BSU_07640
pI
8.67
mw
10.18 kDa
protein length
91 aa Sequence Blast
gene length
276 bp Sequence Blast
function
unknown
product
acylphosphatase
essential
no
ec
3.6.1.7
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    837,735 → 838,010

    The protein

    Catalyzed reaction/ biological activity

  • acyl phosphate + H2O --> carboxylate + H+ + phosphate (according to UniProt)

    • Protein family

  • acylphosphatase family (single member, according to UniProt)

    • Structure

  • [PDB|2FHM] (from ''Bacillus Subtilis'', 100% identity)
  • [PDB|3BR8], [PDB|2FHM] [Pubmed|20447399]

    • Expression and Regulation

    Operons

    genes
    [gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL]
    description
    [Pubmed|22383849]

    additional information

  • 'yflL' transcription occurs in antisense orientation as compared to the surrounding '[protein|B51ADFB53CA54309D866BB089502639E9571238B|Nos]-[protein|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|YflK]' operon
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    Biological materials

    Mutant

  • MGNA-C334 (yflL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2332 NBRP B. subtilis, Japan]
  • BKE07640 (Δ[gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAACCACCTTTTC, downstream forward: _UP4_GGCCATCATCGATTTTCTAT
  • BKK07640 (Δ[gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAACCACCTTTTC, downstream forward: _UP4_GGCCATCATCGATTTTCTAT

    • References

  • 20447399